NASC Stock Detail Page
NASC ID: N850269
Name: WiscDsLox288F06
ABRC stock number: CS850269
Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line. Associated Polymorphisms: Wiscseq_DsLox288F06.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G24620.1 and AT2G24625.1 (chr 2 pos 10465325 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG797375. Wiscseq_DsLox288F06.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G68210.1 and AT1G68220.1-P355 (chr 1 pos 25569769 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG797374. Wiscseq_DsLox288F06.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT2G24255.1 (at chr 2 pos 10317396 (C/10317396-10317480) on the TAIR10). The sequences' 1-84 bps mapped on the genome, 86-100 bps onto the T-DNA. Pools P 1, R 2, C 1 N 1. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO452093. Wiscseq_DsLox288F06.3 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G58550.2-P355 and AT5G58560.1-P355 (chr 5 pos 23670029 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG797376.
Donation Date: 2004-12-01
Donated by: | University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman |
Donor Number: WiscDsLox288F06
Part of : Wisconsin DsLox T-DNA lines
Stock type: individual line
Material type: seed
Status | Price (£) |
---|---|
Available to order at NASC | £11.00 |
Germplasm Info
Mutagen: T-DNA Insertion
Background: Col-2
Transformation method: Agrobacterium
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
---|---|---|---|
|
|
|